primary antibodies against syp  (Proteintech)

Journal: AIDS (London, England)

Article Title: Plasma soluble CD163 is associated with postmortem brain pathology in human immunodeficiency virus infection

doi: 10.1097/QAD.0000000000001425

Figure Lengend Snippet: Multivariable regression results for plasma sCD163.

Article Snippet: Free-floating tissue sections were immunostained with mouse monoclonal primary antibodies against MAP2 (clone 5F9, # 05-346, EMD Millipore, Billerica, MA, USA, 1:20) or SYP (clone SY38, # MAB5258, EMD Millipore, Billerica, MA, USA, 1:10).

Techniques: Clinical Proteomics

Journal: Biochimica et biophysica acta

Article Title: Sex-determining region Y-box 9 acts downstream of NADPH oxidase to influence the effect of leptin on PPARγ1 expression in hepatic stellate cells.

doi: 10.1016/j.bbadis.2016.09.001

Figure Lengend Snippet: Fig. 6. Leptin increases the levels of 4-HNE, p47phox, DLK1, and Sox9 in HSCs in ob/ob mouse model of TAA-induced liver injury. Ob/ob mice were divided into two groups (6 mice/each group) and were, respectively, given administration of TAA (200 μg/g body weight, two times a week) plus vehicle (T + V) or TAA plus leptin (1 μg/g body weight, once per day) (T + L) by intraperitoneal injection for 4-wk. Double fluorescence staining on liver section was performed for detecting positive HSCs for 4-HNE, p47phox, DLK1, Sox9, and synaptophysin (SYP, a marker for quiescent and activated HSCs) by using the respective primary antibody and subsequently the DyLight594-conjugated secondary antibody (red fluorescence) and DyLight488-conjugated secondary antibody (green fluorescence). The nuclei were counterstained with Hoechst 33342 (blue fluorescence). The representative images were captured with the fluorescence microscope. Scale bar 25 μm. Arrowheads indicated examples of positively stained cells. The total HSCs (SYP-positive HSCs) and 4-HNE-, p47phox-, DLK1-, or Sox9-positive HSCs were counted in six randomly chosen fields at 100-fold magnification. The value was expressed as fold change relative to the respective total HSCs (empty column) and was shown as a histogram on the respective right panel. ⁎P b 0.05.

Article Snippet: Briefly, after blocked with normal serum, the liver sections were incubated with primary antibody against p47phox (1:100, Cat. SC-14015, Santa Cruz, CA, USA), DLK1(1:50, Cat. SC-25437, Santa Cruz, CA, USA), Sox9 (1:50, Cat. SC-20095, Santa Cruz, CA, USA), β-catenin (1:100, Cat. SC-7199, Santa Cruz, CA, USA), 4-HNE (1:50, Cat. Ab46546, Abcam, Cambrige, MA, USA), PPARγ (1:100, Cat. 19481, Abcam, Cambrige, MA, USA) and primary antibody against synaptophysin (SYP, 1:10, Cat. SC365488, Santa Cruz, CA, USA), a marker for quiescent and activated HSCs [23], followed by incubationwith DyLight594-conjugated secondary antibody (1:500, Cat. GtxRb-003-D5948NHSX, ImmunoReagents, Inc., Raleigh, USA) and DyLight488-conjugated secondary antibody (1:500, Cat. GtxMu-003-D488NHSX, ImmunoReagents, Inc., Raleigh, USA).

Techniques: Injection, Staining, Marker, Microscopy

Journal: Biochimica et biophysica acta

Article Title: Sex-determining region Y-box 9 acts downstream of NADPH oxidase to influence the effect of leptin on PPARγ1 expression in hepatic stellate cells.

doi: 10.1016/j.bbadis.2016.09.001

Figure Lengend Snippet: Fig. 7. Blockade of leptin-induced NADPH oxidase pathway reduces the productions of 4-HNE, DLK1, β-catenin, and Sox9, accompanied with the increase in PPARγ expression, in HSCs in ob/ob mouse model of TAA-induced liver injury. Two groups of mice (6 mice/each group) were, respectively, received DPI (1 μg/g body weight, once per day) or vehicle throughout the 4- week period of TAA (200 μg/g body weight, two times a week) plus leptin (1 μg/g body weight, once per day) treatment (Two groups: T + L + DPI; T + L + V). Double fluorescence staining on liver section was performed for detecting positive HSCs for 4-HNE, DLK1, β-catenin, Sox9, PPARγ, and synaptophysin (SYP) by using the respective primary antibody and the respective secondary antibody as described in Fig. 6. The nuclei were counterstained with Hoechst 33342 (blue fluorescence). The representative images were captured with the fluorescence microscope. Scale bar 25 μm. Arrowheads indicated examples of positively stained cells. The total HSCs (SYP-positive HSCs) and 4-HNE-, DLK1-, β-catenin-, Sox9-, PPARγ- positive HSCs were counted in six randomly chosen fields at 100-fold magnification. The value was expressed as fold change relative to the respective total HSCs (empty column) and was shown as a histogram on the respective right panel. ⁎P b 0.05.

Article Snippet: Briefly, after blocked with normal serum, the liver sections were incubated with primary antibody against p47phox (1:100, Cat. SC-14015, Santa Cruz, CA, USA), DLK1(1:50, Cat. SC-25437, Santa Cruz, CA, USA), Sox9 (1:50, Cat. SC-20095, Santa Cruz, CA, USA), β-catenin (1:100, Cat. SC-7199, Santa Cruz, CA, USA), 4-HNE (1:50, Cat. Ab46546, Abcam, Cambrige, MA, USA), PPARγ (1:100, Cat. 19481, Abcam, Cambrige, MA, USA) and primary antibody against synaptophysin (SYP, 1:10, Cat. SC365488, Santa Cruz, CA, USA), a marker for quiescent and activated HSCs [23], followed by incubationwith DyLight594-conjugated secondary antibody (1:500, Cat. GtxRb-003-D5948NHSX, ImmunoReagents, Inc., Raleigh, USA) and DyLight488-conjugated secondary antibody (1:500, Cat. GtxMu-003-D488NHSX, ImmunoReagents, Inc., Raleigh, USA).

Techniques: Expressing, Staining, Microscopy